Protein_Domain

Part:BBa_K4643004:Design

Designed by: Baptiste Rivoirard   Group: iGEM23_Sorbonne-U-Paris   (2023-09-24)


pBBR1 Replication protein


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

They were no special design considerations


Source

The replication protein RepB for the broad-host-range plasmid pBBR1 does not originate from an organism but is instead a synthetic or engineered protein specifically designed for the plasmid. The pBBR1 plasmid is a laboratory construct, and its replication protein RepB is not derived from a naturally occurring organism. It is engineered to work effectively in a wide range of bacterial hosts, which is why it is referred to as a "broad-host-range" plasmid. Researchers have designed RepB to initiate and control replication within the plasmid, enabling it to replicate autonomously in different bacterial species.

References